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KMID : 0383119530010030035
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Journal of Aerospace Medicine
1953 Volume.1 No. 3 p.35 ~ p.37
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Abstract
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Macchiavellos oroginal staining method for rickettsial bodies are modified as follows;
1st solution
0.5% Fuchsin distilled water solution
2nd solution
A. 0.5% Citric acid distilled water solution
B. 0.5% Methylene blue distilled water solution
Dilute B solution 10 times with A solution.
The mixture keeps well.
1. Prepare smears as thinly and uniformly as possible and fix by gently heating
2. Stain the preparation for 2-3 minutes with 1st solution
3. Wash with water
4. Apply 2nd solution till the preparation becomes blue
5. Wash with water, dry and microscopy.
This modification is very satisfactory for routine work for demonstration of rickettsial bodies.
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